| Will Arnett | BoJack Horseman / Butterscotch Horseman (voice) |
| Aaron Paul | Todd Chavez / AA Bird / Donkey Kid / Skippy (voice) |
| Alison Brie | Diane Nguyen / Vincent Adultman / Kevin / Samantha The Food Critic / Olivia / Joelle Clark / Otter / Cow Waitress / Sarah Lynn's Assistant / Ship Co-Captain / Golden Snowflake Announcer / Nurse / Waitress / Cow Waitress / Announcer (voice) |
| Amy Sedaris | Princess Carolyn / Sharona (voice) |
| Paul F. Tompkins | Mr. Peanutbutter / Marv / Virgil Van Cleef / Prescott / Andrew Garfield / Sandro / Dog Valet / Underwater Man / Concerned Man / Janitor / Bartholomew Scagsworth / Sloth Lawyer / Blimp Co-Pilot / Abortion Doctor / George Tickle / Giant Chocolate Oscar Guy |
| Aparna Nancherla | Hollyhock Manheim-Mannheim-Guerrero-Robinson-Zilberschlag-Hsung-Fonzerelli-McQuack (voice) |
| Andre Braugher | Woodchuck Coodchuck-Berkowitz (voice) |
| Kelen Coleman | Woodchuck's Advisor / Dr. Picarello / Crowd Member (voice) |
| James Adomian | Stuart (voice) |
| Rami Malek | Flip McVicker / Jose Guerrero (voice) |
| David S. Jung | Gregory Hsung (voice) |
| Jay Pharoah | Dashawn Manheim (voice) |
| Kimiko Glenn | Stefani Stilton (voice) |
| Wendie Malick | Beatrice Horseman (voice) |
| J.K. Simmons | Lenny Turtletaub (voice) |
| Natalie Morales | Yolanda Buenaventura (voice) |
| Keith Olbermann | Tom Jumbo-Grumbo (voice) |
| Jessica Biel | Jessica Biel (voice) |
| Lake Bell | Katrina Peanutbutter (voice) |